Three-phase bone scan (3PBS) and Doppler ultrasound in the assessment of blood flow pattern in repetitive strain injuryAn abstract is unavailable. This article is available as a PDF only.doi:10.1097/00006231-199511000-00042Al-NahhasA.Jawad
The World Health Organization (WHO) defines osteoporosis as bone mineral density (BMD) values of 2.5 standard deviations below the mean for young white adult women1. More recently, a consensus has been achieved in defining osteoporosis not solely based on BMD values, but also as a skeletal diso...
To investigate the osteogenic differentiation potential of MSCs, the MSCs from each donor at passages 3–5 were trypsinized and cultured with a complete DMEM medium in a 35-mm2 dish at a density of 5 × 103 cells/cm2 overnight. Subsequently, the cells were washed with PBS, and osteog...
The actin ring density was quantified by confocal microscopy. BMM, bone marrow-derived macrophages; LPS, lipopolysaccharide. Full size image CCL19 and CCL21 stimulate osteoclasts via the Rho–ROCK signaling pathway Active RhoA GTPase is crucial for cell migration, and CCR7 activates RhoA GTPase in ...
Different bone problems include low bone density, osteoporosis, and other bone diseases. Such bone diseases, ailments, and malfunctions often require complex and expensive treatments. In this study, we synthesized a new type of DLP resin for 3D printing purposes based on poly(ethylene glycol ...
After erythrocytes were removed by lysis, BMM cells were washed, scored, and resuspended at a density of 5 x 106 cells/ml in cold PBS containing 2% FBS. Fluorescent conjugate antibodies were used to stain the BMMs. Antibodies against CD3-FITC (eBioscience, cat# 11-0032-82, 1:200), ...
PBS to remove the phenol red and serum, and reagent solution was added. After incubation for 30 min in a CO 2 incubator, the samples were observed using an inverted fluorescence microscope (DM IL LED Fluo; Leica Microsystems, Wetzlar, ...
Image slices were also reconstructed using cone-beam reconstruction software based on the Feldkamp algorithm (Dataviewer; Skyscan, Belgium). Values of bone-specific parameters, namely bone volume (BV, mm3), bone volume percentage (BV/TV, %), bone mineral density (BMD; g/cm3), trabecular ...
while anabolic drugs while anabolic drugs include the teriparatide (parathyroid hormone (PTH) 1-34), abaloparatide (PTH-related protein (PTHrP) analog), and romosozumab [3,4]. Several therapeutic agents for osteoporosis increase bone mineral density (BMD) and reduce the risk of skeletal fracture...
h, i The protein levels of LATS1, p-LAST1, YAP, p-YAP, TAZ, and p-TAZ, j ALP activities expressed in optical density (OD) values at 520 nm, k–m Runx2 mRNA and protein levels in BM-MSCs transfected with LAST1 overexpression vector/empty vector and treated with mDC-Exo or PBS....