Intrabodies, antibodies expressed within cells, offer an interesting way to target intracellular molecules, making them potentially useful for biotechnology and medicine. However, it remains controversial whether full-size IgG intrabodies expressed in th
The results exhibited that two fusion proteins, Sumo- nClu and Trx-nClu had not obvious expression. Mean- while, GST and NusA had poor solubilization effect on nClu, and the soluble recombinant protein accounted for less than 30% of the total recombinant protein. The effect of MBP was ...
Different aliquots of solubilized inclusion bodies were incubated with 0.2 mL of 2% (w/v) Eudragit L-100 (final concentration, 0.2%, w/v) for CcdB-M97K and malETrx and 0.3 mL of 2% (w/v) cationic starch (final concentration, 0.3%, w/v) for MBP264D, and the final volume was mad...