Briefly, mouse brains were homogenized with micro tissue grinders (Kimble) in 2 ml tubes, using lysis buffer containing 50 mM Tris pH 7.5, 150 mM NaCl, 5 mM EDTA, pH 8.0, 1% SDS, and protease inhibitor (Complete Mini; Roche). After 1 min homogenization, cellular debris was ...