用于BN-PAGE凝胶实验,用于蛋白复合物及亚基研究,常用于分离膜蛋白复合物,胶内活性检测和蛋白质相互作用等领域研究。 使用说明 将上样缓冲液与待测样品液按照1:1比例稀释混匀后,短暂离心取上清进行上样。(无需加热),另行准备毛地黄皂苷,根据实验要求加入相应体积的该试剂后进行电泳。
用于BN-PAGE凝胶实验,用于蛋白复合物及亚基研究,常用于分离膜蛋白复合物,胶内活性检测和蛋白质相互作用等领域研究。 使用说明 本品为20倍浓缩液,使用前请用双蒸水稀释至1倍后再进行电泳实验并请根据BN-PAGE一向和二向电泳相关资料要求进行使用 组分 阳极缓冲液(Bis-Tris,pH 6.9~7.1)阴极缓冲液(Tricine,Bis-Tris...
BN-PAGE实验操作步骤BN-PAGE实验操作步骤.doc,Supplemental Material Appendix S1. Experimental procedures Pigment analysis Prior to pigment extraction, each sample was weighed. Seedlings were frozen in liquid nitrogen and ground to powder with a high-speed m
BN-PAGE实验操作步骤 Supplemental Material Appendix S1. Experimental procedures Pigment analysis Prior to pigment extraction, each sample was weighed. Seedlings were frozen in liquid nitrogen and ground to powder with a high-speed mixer mill (model MM301, Retsch, http://www.retsch.com). Tot...
BN-PAGE实验操步骤.doc,Supplemental Material Appendix S1. Experimental procedures Pigment analysis Prior to pigment extraction, each sample was weighed. Seedlings were frozen in liquid nitrogen and ground to powder with a high-speed mixer mill (model MM30
BN-PAGEMultiprotein ComplexesCell SignallingProtein-protein Interaction NetworksNative ConditionsResolutionGel FiltrationSucrose Density UltracentrifugationMPC SizeCompositionRelative AbundanceHydrodynamic Size And ShapePolyacrylamide MatrixCellular LysatesLysate DialysisFirst Dimension BN-PAGESecond Dimension SDS-PAGE...
BN_PAGE在分析蛋白质中的应用_蔡家海
BN-PAGEMultiprotein ComplexesCell SignallingProtein-protein Interaction NetworksNative ConditionsResolutionGel FiltrationSucrose Density UltracentrifugationMPC SizeCompositionRelative AbundanceHydrodynamic Size And ShapePolyacrylamide MatrixCellular LysatesLysate DialysisFirst Dimension BN-PAGESecond Dimension SDS-PAGE...
Native-PAGE实验方法与问题及其解答 主要内容: 分离酸性蛋白步骤 ... 热度: SDS-PAGE胶 制作步骤 热度: 1 SupplementalMaterial AppendixS1.Experimentalprocedures 5Pigmentanalysis Priortopigmentextraction,eachsamplewasweighed.Seedlingswerefrozenin liquidnitrogenandgroundtopowderwithahigh-speedmixermill(modelMM301,Retsc...
(USB, http://.usbweb) using BSA as a standard, then separated by 10% SDS-PAGE. The proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane (Bio-Rad, http://discover.bio-rad) by electroblotting for...