Molecularly, we identify that primary cilia endow endothelial cells with strongly enhanced sensitivity to bone morphogenic protein 9 (BMP9), selectively under low flow. We propose that BMP9 signaling cooperates with the primary cilia at low flow to keep immature vessels open before high shear ...
转染的细胞用G418筛选 ,利用斑点杂交和免疫组化检测转染 4周后外源基因表达的情况.结果 :琼脂糖电泳证明mBMP 4真核表达载体 pCR 3 .1uni mBMP 4构建成功.细胞转染后第 4周 ,斑点杂交和免疫组化均显示为阳性 ,阳性细胞碱性磷酸酶表达升高.结论 :外源BMP 4基因可在成纤维细胞内获得表达并可影响靶细胞的表现...