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Five tetraploid potato cultivars, namely Agria, Jelly, Karlena (drought-tolerant) [73], Kolibri (drought-tolerant) [73] and Laura (drought-sensitive and heat-tolerant) [74], were grown in plant growth chambers (Fitotron SGC 120 Humidity, Weiss Technik GmbH, Germany) in 1.5 L pots using a...
Swiss Academic Software GmbH. Citavi (6); 2020. https://www.citavi.com. Ouzzani M, Hammady H, Fedorowicz Z, Elmagarmid A. Rayyan-a web and mobile app for systematic reviews. Syst Rev. 2016;5:210. https://doi.org/10.1186/s13643-016-0384-4. Article PubMed PubMed Central Google Sch...
(CLN GmbH, Germany). In a 1.5 ml Eppendorf tubes, a few colonies were selected and 200 μl Tris EDTA (TE) was added, then the tubes were subjected to overnight freezing. A volume of 200 μl of freshly prepared chloroform: methanol mixture was added to the tubes after thawing ...
containingporM2and two other ORFs. Upstream toporM2a -10 signal of a promoter (TACGTT), a ribosome binding site (AGGGAGAA) and a signal peptide recognition sequence (SP) of 93 bp were identified. Subsequences were predicted using the software packages MacVector™ 7.2.3 (Accelrys) and ...
On the other hand, the RegionMiner tool of the Genomatix suite (Genomatix Software GmbH, Munich, Germany) identified the most over-represented motifs, based on the background of occurrences of the transcription factor binding sites (TFBSs) within the whole sequence of the rat genome (rn4; ...
4’,6-diamidino-2-phenylindole (DAPI) (Ex-Max 358 nm/ Em-Max 461 nm)(cat nr. 10,236,276,001, Roche Diagnostics GmbH, Germany) was used to discriminate dead from live cells. Concentration was adjusted to 105–106 cells/ml, and antibody added at concentration of 1 μl per 105–106 ...
LifeGlimmer GmbH, Markelstrasse 38, 12163, Berlin, Germany Erno Lindfors, Carolyn Ming Chi Lam & Vitor A. P. Martins dos Santos Laboratory of Systems and Synthetic Biology, Wageningen University & Research, Stippeneng 4, 6708 WE, Wageningen, The Netherlands Jesse C. J. van Dam, Niels A....