BM-hMSCs)增殖及成骨分化的影响.方法:从ScienCell实验室购买的BM-hMSCs分别在无血清生长培养基(对照组)和添加4mmol(4P组)、8mmol(8P组)磷离子的无血清生长培养基中培养21天,通过CCK8比色法评估细胞的增殖情况;RT-PCR检测成骨分化标记性基因胶原蛋白Ⅰ、骨钙素、碱性磷酸酶的表达水平;茜素红染色检测BM-h...
four different cell populations were monitored: a) control BM-hMSCs grown in Maintenance Medium (MM) up to 27 days without exposure to PEMF (ODM−/PEMF−); b) cells grown in MM up to 27 days and daily exposed to PEMF for 10 minutes (ODM...