Purpose To investigate the impact of BM-MDSCs on murine heart transplantation. Material and Methods Bone marrow cells were harvested and cultured with granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-6 (IL-6) for 4days to generate Gr1+ CD11b+ BM-MDSCs.Vascularized ...
Comparison of suppressive activity of MDSCs with BM-derived IMCs.Parvin, ForghaniWayne, HarrisCynthia, R. GiverAbbas, MirshafieyJacques, GalipeauEdmund, K. Waller
In ex vivo suppression assays, M-MDSCs, enriched from spleens of LP-BM5 infected mice inhibited both T- and as a novel finding, B-cell responsiveness. For M-MDSC suppression of T-cell proliferation and IFN-gamma production, we previously reported the mechanism to be almost completely iNOS/...
Here, we treated LP-BM5 infected mice with an extended course of 5-FU: at 6–7 weeks post infection achieving ~50% depletion of splenic M-MDSCs, and spleen cells from depleted mice exhibited greatly decreased suppression in ex vivo assays. LP-BM5 infected mice with this level of M-...
Phenotype and morphology of myeloid-derived suppressor cell (MDSC)-like cells generated in vitro from murine bone marrow (BM) in comparison with synovial fluid (SF) cells.doi:10.1371/journal.pone.0111815.g001K. JuliaVida ...