THP-1 cells attached on the upper side of the filter were gently removed with a cotton swab. THP-1 cells that migrated through the filters of the 96-well permeable supports were quantified by measuring relative fluorescence units with a plate reader (SpectraMaxi3x, Molecular Devices, LLC, San...
To perform the experiment, overnight culture in a stationary growth phase was diluted to 0.5 McFarland in sterile PBS. For samples non-treated with photoinactivation (control), bacteria were spread with a cotton swab on the solid plates containing the Mueller Hinton Agar (MHA) (Sigma Aldrich,...
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The increased prevalence of multidrug-resistant organisms (MDROs), healthcare associated infections (HAIs), and the recent COVID-19 pandemic has caused the photoinactivation industry to explore alternative wavelengths. Blue light (BL405) has gained signi