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The bound protein was eluted in HBS containing 0.1% (w/v) LMNG, 0.01 mM TCEP (pH 7.0), and 250 mM imidazole (pH 8.0) and further purified over an in-house anti-FLAG M1 affinity Sepharose column as follows. The eluate was supplemented with 2 mM CaCl2 before loading over the anti-...
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