To further evaluate the importance of smoking status in age prediction we included smoking status as an input feature along with blood test values and trained the new set of DNNs on the three extended sets of input features. Smokers were included in the training set for this round. To robustl...
First, we reanalyzed public human blood methylation data to determine the trajectory of TE methylation during aging, comparing evolutionarily young and old TEs. We then constructed age predictors for mice and humans. Lastly, we investigated associations between accelerated age prediction, and more ...
With the increasing incidence of breast lesions, the differential diagnosis between benign lesions and breast cancer (BCa) has become a big challenge. Host peripheral blood mononuclear cells (PBMCs) could undergo changes in DNA methylation upon disease p
3d). Alteration of the regulation of some of these genes might therefore determine blood-cancer pleiotropism. The candidates include five HLAs and the major histocompatibility complex (MHC) class I polypeptide-related sequence A (MICA) genes. Pleiotropic factors are frequent regulators of hematopoiesis...
We also profiled mutations and methylation changes in parallel from the pre-MCP library by targeted sequencing (RACEseq). To determine the RACEseq panel for cfDNA profiling, we selected potential methylation markers based on the publicly available datasets from The Cancer Genome Atlas (TCGA) and ...
A blood-based non-invasive screening test for CRC using cell-free DNA (cfDNA) methylation sequencing is developed here from the blood of 212 controls and 67 treatment naive CRC patients from 21 sites across India and processed in Strand's reference lab in Bangalore.Methods:Steps involved cfDNA ...
Liquid biopsies are already used to guide treatment of people with confirmed cancer. They help monitor the spread of cancer to other parts of the body; identify a tumor's genetic changes, or mutations; determine what drug treatments might work best for specific patients; and reveal whether treat...
Studies have linked methylation at many genomic loci to smoking status [24,25,26,27,28,29]. Therefore, to adjust for DNAm differences related to smoking, we calculated smoking scores from DNAm data based on the weights obtained from 39 CpGs located at 27 loci, as previously described [30...
To better understand DNA methylation in Alzheimer’s disease (AD) from both mechanistic and biomarker perspectives, we performed an epigenome-wide meta-analysis of blood DNA methylation in two large independent blood-based studies in AD, the ADNI and AIB
To determine whether the altered DNA methylation atCTSZwe observe in blood cells is present in the pulmonary endothelium, we queried public DNAme data in pulmonary endothelial cells from PAH samples (Methods). These demonstrated a trend towards hypermethylation at theCTSZCpG cg04917472 (p-value =...