These properties, coupled with a functional brain endothelial expression profile and the capability to easily test several repeats with low reagent consumption, make this BBB model highly suitable for widespread use in academic and industrial laboratories....
An Excel spreadsheet was used to record inclusion or exclusion decisions, along with specific reasons for exclusion when applicable. Discrepancies at this stage were resolved through discussion between reviewers, and in cases of lack of consensus, a fourth reviewer was consulted for the final decision...
QC of the libraries was conducted, with the following three steps: (1) preliminary test of the library concentration using Qubit 2.0, (2) test of the insert size using Agilent 2100, and (3) precise quantification of the effective concentration of library using qPCR. The libraries were ...
The tail suspension test (TST) was conducted on the 23rd day postpartum in a quiet location. Mice were suspended by their tails using adhesive tape approximately 30 cm above the tabletop for 6 min, and the immobility time of the mice was recorded during the following 4 min. The for...
Significant outliers were determined using the intensity-based Significance B option (two-sided Benjamini-Hochberg test) with a FDR cut-off set to 0.05. The protein list was filtered for reverse hits, proteins that are only identified by site and potential contaminants. In addition, proteins ...
3.4. Test of residual antibiotics To detect potentially residual antibiotics in the porcine whole blood, four different types of antibiotic, chloramphenicol, quinolones, sulfonamides, and tetracyclines, were analyzed using the enzyme-linked immunosorbent assay (ELISA) (Fig. 3). Commercialized antibodies ...