time polymerase chain reaction (PCR) platform used as a laboratory-developed test (LDT) for the detection of HSV-1 and -2 in whole blood (WB).MethodsA clinical comparison study comparing a real-time PCR reference assay to a LDT based on the DiaSorin Simplexa Direct assay kit was ...
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Concomitantly, HIV-infected CSWs presented a dysregulated blood B-cell compartment, characterized by increased total IgG1, increased frequencies of populations presenting immature and/or innate profiles and a higher ratio of IgG+/IgA+plasmablasts. In contrast, relatively low levels of BLyS in the blo...
[39] make use of color space transformation using two-color spaces, cyan–magenta–yellow-key (CMYK) and hue-saturation-value (HSV), along with Otsu’s thresholding to segment the blood cell nuclei for feature extraction. Kutlu et al. [32] exploited R-CNN and transfer learning to obtain ...
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But this paper addresses WBC-type detection using four popular color spaces – RGB (red, green, and blue), HSV (hue, saturation, and intensity), YCbCr (luminance, chroma blue, and chroma red), and L*a*b* (luminosity, chromaticity layer "a*", and chromaticity layer "b*"). The ...
3B, this cropped region is further converted from the RGB (red, green, blue) channel to HSV (hue, saturation, value) space, and the saturation channel is extracted, which provides the maximum intensity contrast between the fluorescent labelled WBCs and the background. Next, as seen in FIG....
1c). The following diagnostic tests were performed in all MS patients to exclude differential diagnoses: polymerase chain reaction (PCR) for cytomegaly virus, Ebstein-Barr virus, Human Herpes Virus-6, Herpes simplex Virus (HSV)-1, HSV-2, and Varicella-Zoster Virus in CSF. Blood tests for ...
(HSV1, HSV2, EBV, CMV, HHV6, HHV7, HHV8), the polyoma viruses (BK/JC virus) may be more difficult to detect. This is because serological assays are often of little use in this setting, as IgG and IgM assays, or even avidity assays, are not accurate because immune suppression ...
“A blood test for Alzheimer’s disease could be administered easily and repeatedly, with patients going to their primary care office rather than having to go into a hospital,” commented corresponding author Dominic Walsh. “Ultimately, a blood-based test could replace cerebrospinal fluid testing ...