The ACT Plus (formerly Hemotec [Hepcon] ACT) device is a cartridge with two chambers that contain kaolin activator and are housed in a heat block. Blood (0.4 mL) is placed into each chamber, and a daisy-shaped plunger is raised and passively falls into the chamber. The formation of...
Antinuclear antibodies (ANA) can be detected by the same technique using human epithelial cell line HEp-2 as a substrate. Read more View chapterExplore book Immune reconstitution: an important component of a successful allogeneic transplantation Katalin Paloczi, in Immunology Letters, 2000 Serum ...
using a dot blot assay with antibodies against human SA (HSA) to find that Pt NPs are indeed coated with SA (Fig.1e). We then analyzed the Pt NPs by mass spectrometry (MS). The surface protein fragments were unique fragments of HSA (Fig.1f). To test if Pt NPs could be synthesized...
In several different cell types (MCF10A, HepG2 and UOK262) blocking glucose metabolism, or shifting cellular metabolism away from glycolysis was shown to impair YAP/TAZ transcriptional activity and its ability to promote cell proliferation, cancer cell self-renewal and clonogenic potential in vitro...
it is thawed before use and has a shelf life of 1 year. Interestingly, while O- is the preferred universal donor for blood, the plasma of AB blood groups is the most preferred because their plasma does not contain antibodies, making it acceptable for everyone without fear of an adverse rea...
Total antibody to hepatitis B core antigen (anti-HBc):This test measures antibodies that show if you have ever had an active HBV infection. Anti-HBc appears at HBV infection onset and persists for life. IgM antibody to hepatitis B core antigen (IgM anti-HBc):This test shows whether an acu...
120 donors, negative for anti-HCV and anti-HIV antibodies, were analyzed. All recruited donors were unremunerated volunteers from either urban or rural areas. They were medically assessed and via a questionnaire denied any known risk factors for viral infection. Donors found to be HBV carriers we...
In this study, five HIV-infected patients who tested positive or indeterminate for anti-HEV IgG antibodies and negative for HEV RNA, presented a positive or indeterminate test for the presence of anti-HEV IgM antibodies by EIA method. Of these, three were confirmed positive by IB method, thus...
Hepatitis CBlood TransfusionHepatitis C AntibodiesProspective StudiesAdultAgedAged, 80 and overChildChild, PreschoolIn November 1989, the Japanese Red Cross began screening blood donors for the hepatitis C virus antibody (anti-HCV) by first-generation assay and high-titer hepatitis B virus core ...
(150 μg/ml) or in the human hepatocellular liver carcinoma cell line or HepG2 cell line (ATCC, Manassas, Va., USA). The mRNA expression levels of the coagulation factors VII and X and prothrombin were measured according to the protocols of real-time quantitative RT-PCR with ABI Prism ...