c-Fos cRNA probe (red) was used in conjunction with nuclear DAPI staining (blue). Abbreviations: d, dorsal region; vl, ventrolateral region. n = 3 for virgin female-Hb, n = 4 for male-control and Hb, virgin female-control, n = 6 for mother-control, and n = 7 ...
and the green phase was subtracted from the red phase to enhance contrast; d) contrast and shading were optimized again in the subtracted image; e) to detect the vessels, the region of interest (ROI) was first set along the limbus (red circle); f) the gray value range was then adjusted...
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