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The height of these structures is 5 μm and 50 μm in height, respectively. A mixture of a base and a curing agent with a 10:1 weight ratio (SYLGARD 184 A/B, Dowcorning, Midland, MI) was poured onto the SU-8 mold and cured at room temperature under vacuum for one hour and...
The presence of the lactic acid, among other things, contributes to the continued decrease in the pH of the stored whole blood. The lowering of the blood pH has a dramatic effect on the viability of the erythrocytes when the blood is transfused. The mechanisms involved in the effect are...
dependent on both field frequency and cellular electrical properties26. The DEP system employed here measures the response of ~20,000 cells per test, using 20 different frequencies in parallel27(Fig.1a), and has been used in a range of cellular applications including drug ...
Challenges in drug development of neurological diseases remain mainly ascribed to the blood–brain barrier (BBB). Despite the valuable contribution of animal models to drug discovery, it remains difficult to conduct mechanistic studies on the barrie
(SYLGARD 184 Silicone Elastomer, Dow Corning Corporation, Midland, MI, USA) were mixed in a 10:1 ratio and PDMS chips were molded from the wafer. After an intermediate cleaning step, the PDMS chip and a glass substrate were surface-activated via oxygen plasma and covalently bonded to each ...
Based on the protocol reported in a previous study [22], a polydimethylsiloxane (Sylgard 184, Dow Corning, Midland, MI, USA) microfluidic device was fabricated using the soft lithography technique. To stabilize the pulsatile blood flows generated by the micropump, an air damper was connected to...
(SYLGARD 184 Silicone Elastomer, Dow Corning Corporation, Midland, MI, USA) were mixed in a 10:1 ratio and PDMS chips were molded from the wafer. After an intermediate cleaning step, the PDMS chip and a glass substrate were surface-activated via oxygen plasma and covalently bonded to each ...
PDMS replicas were obtained by curing a degassed mixture of Sylgard 184 Silicone Elastomer Base and the Curing Agent 10:1 (Dow Corning, Midland, TX, USA) poured onto both molds and cured at 65 °C, 4 h in an oven. After separating the PDMS replica from the mold, inlet and outlet hole...
(CC, width = 400 µm), and an outlet. The depth of all the channels was fixed at 50 µm. Based on the protocol reported in a previous study [22], a polydimethylsiloxane (Sylgard 184, Dow Corning, Midland, MI, USA) microfluidic device was fabricated using the soft lithography ...