When investigating immune responses in infections or diseases in humans, the most relevant or important tissues are not usually readily accessible, which often limits these types of studies. Sampling of peripheral blood 1Malaria Immunology laboratory, Francis Crick Institute, 1 Midland Road, NW1 1AT...
The height of these structures is 5 μm and 50 μm in height, respectively. A mixture of a base and a curing agent with a 10:1 weight ratio (SYLGARD 184 A/B, Dowcorning, Midland, MI) was poured onto the SU-8 mold and cured at room temperature under vacuum for one hour and...
Some cross-sections were opened longitudinally, and some of these were pinned onto gauze (NuGauze; Johnson and Johnson Gateway, Inc., Piscataway, NJ) on top of a 2- to 3-mm layer of Sylgard resin (DowCorning, Midland, MI) in 100-mm tissue culture dishes (Becton Dickinson, Mountain ...
As discussed previously, the vast majority of studies that aimed to assess CSF or blood biomarkers of SCI have done so based on ‘targeted’ proteins that are known to relate to the biological processes that occur following a SCI. Many of these biomarkers have so far been assessed in pre-cl...
Circadian rhythms organize many aspects of cell biology and physiology to a daily temporal program that depends on clock gene expression cycles in most mammalian cell types. However, circadian rhythms are also observed in isolated mammalian red blood cel
A disposable microfluidic chip was fabricated with poly(dimethylsiloxane) (PDMS, SYLGARD 184 silicon elastomer; Dow Corning Co., Midland, MI, USA) attached to a thin glass slide by the same procedure as we reported previously [20], and was used for monitoring and sorting of cells. The design...