The ISS-N1 targeting 20-mer PMO with primary amine at the 3′-end (5′-ATTCACTTTCATAATGCTGG-3′) was purchased from Gene Tools LLC (Philomath, USA). SsoAdvanced Universal SYBR Green Supermix and iScript reverse transcription supermix were purchased from Bio-Rad Laboratories, Inc. (...
SMBS_DPP contained the cata- lytic triad (Ser-633, Asp-711, His-743), a predicted trans- membrane domain at the N-terminus. Phyre2 was able Fig. 4 Aspartic protease of S. molnari – signal peptide peptidase. a Phylogenetic relationship of S. molnari SPPL (Sm_SPPL), (ML, 1000 ...