Isopropyl alcohol (0.5 mL) was added to the transferred aqueous phase, mixed well, and then left to incubate at rt for 10 min before centrifugation at 12000 xg for 10 min at 4 °C to precipitate the RNA. Subsequently, the supernatant was thoroughly removed, and the RNA pellet ...
Myxozoans are entirely parasitic in their life cycle, they alternate between a verte- brate and an invertebrate host with two entirely different types of transmissible spores in each developmental phase [15–18]. Myxospores are often hardy stages that are cap- able of being exposed to the ...