Accuracy of ICD-10 coding for sepsis and organ dysfunctions in children with blood culture-proven sepsis in Switzerland, results from the prospective Swiss Pediatric Sepsis StudyAgyeman PkaPosfay Barbe KmGiancarlo NatalucciKaren Triep
Bone marrow stromal cells can be easily isolated and massively expanded in culture in an undifferentiated state for therapeutic use. Owing to their potential to modulate local micro-environment via anti-inflammatory and immunosuppressive functions, BMSCs have an additional advantage for allogeneic ...
The events will be classified according to the ICD-10 (International Statistical Classification of Diseases, 10th Revision) code associated with the event. In case the ICD codes are not available in the transferred data, the definition of events as given by the investigators will be accepted with...
This 48 h time period was chosen because blood cultures requested overnight may not be regis- tered on the database until the following morning, po- tentially underestimating the frequency of blood culture use in this setting. The admission diagnosis was derived from the primary ICD-code for ...
Cell culture HEK293T cells were cultured in DMEM supplemented with 10% FBS and 1X penicillin/streptomycin. Human CD34+ HSPCs were purchased from Fred Hutchinson Cancer Institute and cultured in StemSpan serum free culture medium (StemCell Technologies) supplemented with 1X CC100, 2mM L-glutamine,...
Human cardiac fibroblasts (HCFs) were purchased from Promocell and cultured in fibroblast basal medium FBM-3 supplemented with 10% fetal bovine serum (FBS), supplements (Promocell), 100 μg/ml penicillin and 100 μg/ml streptomycin under standard cell culture conditions (37 °C, 5% CO2). Tr...
1999, Tokyo, and Rojin-hoken Jigyou-hi tou Hojokin, 1999, Tokyo, from the Ministry of Health and Welfare, and by a Research Grant for Scientific Research (10470102) and a Research Grant for JSPS Research Fellows (01180) from the Ministry of Education, Science and Culture of Japan, Tokyo...
Materials and Methods Plasmodium falciparum culture. Plasmodium falciparum 3D7 was cultured in complete RPMI (1640 (Invitrogen Corporation, USA), 50 mg/L hypoxanthine (Sigma Aldrich Co., USA), 0.5 g/L Albumax I (Gibco, Thermofisher Scientific Inc., USA) and 2 g/L sodium ...
In addition, IL12p70 has been shown to reduce neuronal viability in cell culture experiments, both in the presence or absence of Aβ [29]. Lastly, chronic inflammation leads to elevated CCL4 levels in AD brains [30], while CCL4 levels are also increased in microglia associated with Aβ ...
(23% of participants) had an increase in MAP ≥ 7 mmHg on 300 mEq Na+ intake and a decrease in MAP ≥ 7 mmHg on 10 mEq Na+ intake [24]; and ISS (13% of participants) had a decrease in MAP ≥ 7 mmHg on 300 mEq Na+ intake and an increase in MAP ≥ 7 mmHg on a 10 mEq...