All RNA extractions were performed within two hours of blood collection. Briefly, 7.5 ml of whole heparinised blood was mixed with 42.5 ml of erythrocyte blood lysis buffer (10 mM KHCO3, 150 mM NH4Cl, 1 mM EDTA pH 8.0), and incubated for 5 min at room temperature with gentle agitation...
Venous blood was collected in the morning after an overnight fast; then, one part of venous blood was isolated by centrifugation at 3000 rpm for 10 min within 1 h of sample collection. The separated serum was stored at − 80 °C before evaluation for detecting anti-NMDAR abs. Anothe...
* Elastic groove in holder can lock tube, insure safe blood collection. * Lock Multi-sample needle short end with latex cover directly into holder, insertion finish with a snap. * After collection finished, press button on holder, needle automatically discha...
A study using Sgk1 KO mouse hearts found that the lack of SGK1 impairs in vitro endothelial cell tube formation and reduces blood vessel production after myocardial infarction [30]. Cardiovascular diseases often involve autonomic nervous system abnormalities, such as hypertension, cardiac diseases, ...