We sat on a bench near a broken drinking water fountain. Somebody had driven a peg in its spout, thus solving a plumbing issue. People say that birds don't sing in former battle fields because so many deaths had occurred there. I can make a personal contribution to the topic. Birds ...
For both types of PCR, a negative control (nuclease-free water) and a positive control (extracted DNA from bird blood, infected with Plasmodium (in the investigation of haemosporidian parasites) or Trypanosoma (in the search for tripanodomatids) parasites confirmed both by PCR and microscopy)...
Briefly, plasma samples were allowed to equilibrate at room temperature, and subsequently denatured and diluted with equal parts of methanol and water, then mixed and extracted on C18 solid-phase extraction (SPE) cartridges (JT Baker, Phillipsburg, NJ, USA). Each cartridge was dried and eluted ...