For both types of PCR, a negative control (nuclease-free water) and a positive control (extracted DNA from bird blood, infected with Plasmodium (in the investigation of haemosporidian parasites) or Trypanosoma (in the search for tripanodomatids) parasites confirmed both by PCR and microscopy)...
Thereafter, 1 mL of Milli-Q water and 2 mL of petroleum ether were added to the samples. After vortexing and centrifugation, 3 mL of the organic phase was collected and evaporated at 40 °C under nitrogen gas. 2.4. Analysis of Fatty Acids by GC-MS The fatty acids were separated by ...