A top-down scalable method to produce flexible water and blood repellent tubes is introduced. The method is based on replication of overhanging nanostructures from an aluminum tube template to polydimethylsiloxane (PDMS) via atomic layer deposition (ALD) assisted sacrificial etching. The nanostructured ...
ABO blood groupThe varied distribution of sialic acid-containing receptors on host cells’ surfaces, modulated by ABO antigens, could influence the binding capacity of the virus's spike protein S1 to ACE2 and CD147 of the host cells.[114] ...
Quantification of each of the libraries was done using a Qubit dsDNA HS assay kit and a Qubit fluorometer (ThermoFisher Scientific, Waltham, MA, USA). The concentration of the library was then adjusted to a final concentration of 4 nM using nuclease-free water and applied to the MiSeq ...
To investigate the ovarian responses, ovarian and uterine hemodynamics, circulating ovarian hormones, and nitric oxide (NO) with their relations in superstimulated cows. Eight Holstein Friesian dry cows previously synchronized with CIDR underwent rectal Doppler ultrasound scanning and blood sampling after ...
To this end, an ice-water bath combined with cyclophosphamide was used to prepare a blood deficiency and blood stasis syndrome model. UHPLC-MS technology was used to analyze the metabolites in rat plasma. Through mass spectrometry and public database retrieval, the detected metabolites were ...
Mobile phase A was 0.1% formic acid in water, and mobile phase B was 0.1% formic acid in 90% acetonitrile (LC-MS Chromasolv, Sigma-Aldrich). Partial-loop LC injections of 10 μl corresponded to 107 nL of the original whole blood sample. The flow rate was 0.4 ml/min and the mobile...
Lane 1, hES cells; Lane 2, RT-negative control of the hES cells; Lane 3, USSCs; Lane 4, RT-negative control of the USSCs; Lane 5, day seven spheres; Lane 6, RT-negative control of the day seven spheres; and Lane 7, water control. B, RT-PCR of genes representative of the ...
DNA was extracted using the QIAamp DNA Blood Mini Kit (Qiagen, Redwood City, CA, USA) and eluted into 50 μL of PCR-grade water. Following extraction, DNA concentrations were determined using a Qubit 2.0 Fluorometer with the Qubit dsDNA High Sensitivity Assay Kit (Life Technologies, Grand ...
Materials and methods Animals All experiments were performed in adult male C57BL/6 J mice purchased from Janvier Labs (Saint-Berthevin, Cedex, France). Mice were maintained on a 12 h-light–dark cycle with free access to water and standard chow diet (Altromin, #1314) or 58% high ...
aureus (MSSA ATCC 25923), methicillin-susceptible coagulase-negative Staphylococcus (ATCC 12228) and a negative control with sterile distilled water (DW) were included throughout the procedures. Probes for mecA/meC, nuc and 16S rRNA were detected using channels 530, 560 and 610 respectively. A ...