The concentration of alcohol in the blood, expressed as the weight of alcohol in a fixed volume of blood and used as a measure of the degree of intoxication in an individual. The concentration depends on body weight, the quantity and rate of alcohol ingestion, and the rates of alcohol absor...
Example of the carbapenemase enzyme detection test with reading at 4 h and 6 h, for the Blood-rsCDM. A, imipenem; B, imipenem + APBA; C, imipenem + EDTA; D, imipenem + APBA + EDTA. KPC carbapenemase at 4 h (Figure I), MBL carbapenemase at 4 h (Figure II), ...
Following lysis centrifugation, the whole blood samples of all patients under study were inoculated into Colombia agar base supplemented with 5% defibrinated sheep blood (CBA). The plates were subsequently incubated for a minimum of 21 days at 37 °C in a 5% CO2atmosphere25,26. Results Fifty ...
Amplified products were visualized on a 1% (w/v) agar- ose gel electrophoresis. Data analysis The difference in trypanosome detection between the different sample preparations was analysed using the Chi-squared test (the degrees of freedom were noted as a subscript to the c2 -statistic) and was...
one culture plate with mixed growth (21 h group), located off the inoculation streaks in the 3rd and 4th quadrant of the chocolate agar plateAmplification of genetic material was noted in one blood culture bottle using an assay targeting Staphylococcus spp. Decision to use this assay was made ...
Consistent with this possibility, E-cadherin expression is required for anchorage-independent growth of uveal melanoma cells in soft agar.45 The primitive nature of class 2 melanoma cells may also promote the dissemination and colonization of distant sites, which is reminiscent of the behavior of ...
Tissue processing initially involves ethanol dehydration to remove water, addition of an infiltration agent (e.g., paraffin wax or a resin), which solidifies the medium and then embedding in an external medium (e.g., agar, gelatine) to harden. The hardened blocks containing the tissue samples...
The current culture-based approach for the diagnosis of bloodstreams infection is incommensurate with timely treatment and curbing the prevalence of multi-drug resistant organisms (MDROs) due to its long time-to-result. Bloodstream infections typically involve extremely low (e.g., <10 colony-forming...
The study protocol was approved by the local ethics committee, and an informed written consent was obtained from all the patients/guardians. 2.2. Tonsils and bacterial samples One half of each removed tonsil was ground and cultured on blood agar plates under aerobic conditions. Streptococci were ...
Hand-picked islets where fixed in 2.5% glutaraldehyde in freshly prepared Millonig and post fixed in 1% osmium tetroxide before being dehydrated and embedded in AGAR 100 (Oxford Instruments Nordiska, Lidingo¨, Sweden) and cut into ultrathin sections. The sections were put on Cu-grids and ...