Mitomycin-C: Mitomycin-C is an antineoplastic antibiotic and a cell cycle specific alkylating agent derived from Streptomyces caespitosus. Due to availability of more efficacious regimens, the clinical utility of mitomycin-C in the United States is mostly limited to the treatment of anal cancer. ...
It is these highly reactive intermediates that attack DNA and produce DNA strand breakage and maximum cytotoxicity in the late G2 and early M-phases of the cell cycle. View all Manufacturing Process To a medium having a composition of 6.4 % of millet jelly, 0.5 % of glucose, 3.5 % of ...
In conclusion, mutagen sensitivity is associated with damage-resistant growth, which is indicative of impaired cell cycle arrest. By which specific pathway(s) this checkpoint defect is explained has yet to be elucidated; however, it is probably distinct from the checkpoint defect in AT cells. ...
Figure 7.Representative trichrome imaging of (a) normal mouse lung, (b) intratracheal bleomycin, (c) HPS1 −/− mouse treated with reduced dose of i.t. bleo, and (d) lung-specific, doxycycline overexpression of the bioactive form of the human TGF-β1 gene. ...
Besides that the drug should be a non-cell cycle specific drug as gliomas have a very low growth fraction 5 and should have shown at least some effectivity against glioma cells both in vivo and in vitro 7-9 . Bleomycin fulfilled all these criteria 2, 6 and has been taken as the first...
Either excessive accumulation or depletion of cellular polyamines is harmful to mammalian cells and can lead to cell death48. Polyamines may activate or inhibit apoptosis depending on the concentration and the specific apoptotic inducers. Regarding the possible mechanisms of the antiapoptotic effects, ...
Site-specific cleavage of RNA and DNA by complementary DNA--bleomycin A5 conjugates. Bioconjug Chem. 2003 Nov-Dec;14(6):1307-13. PMID: 14624648. Abraham AT, Lin JJ, Newton DL, et al. RNA cleavage and inhibition of protein synthesis by bleomycin. Chem Biol. 2003 Jan;10(1):45-52. ...
MSC-based therapies have been shown to attenuate various forms of organ damage in preclinical models, both by differentiating into tissue-specific functional cells and by creating a regenerative microenvironment14,15. Beneficial effects of MSCs on bleomycin-induced pulmonary damage have been demonstrated ...
Specific Mmodeling Methods Mice: C57BL/6 • 12-week-oldAdministration: 3-5 mg/kg • intratracheal administration • sprays on day one Note The mice were housed in separate stainless-steel cages (six mice per cage) in a temperature-controlled environment (20-24°C) on 12 h light...
These differences are explainable by the different modes of disease induction, i.e., direct and bronchocentric injury with focal inflammation by Bleomycin, and bronchial epithelium and AT2 cell-specific secretion and spreading of TGFβ1 in the AAV model. Interestingly, despite the strong and ...