调用blastn合作加-help参数可以打印出下面详细的帮助信息 blastn -help blastn [-h] [-help] [-import_search_strategy filename] [-export_search_strategy filename] [-task task_name] [-db database_name] [-dbsize num_letters] [-gilist filename] [-negative_gilist filename] [-entrez_query en...
今天逛论坛看到了,解决办法是指定参数-task,执行网页blast模式,亲测可用。 eg : blastn-querytest.fa-dbdb_blast-evalue1e-6-outfmt6-outblast.res-taskblastn-short
第6步:用blastn搜索outerrepeat,-task blastn,表示somewhat blast,其他均为默认参数 makeblastdb-inreference_LTR_mask.single_letter.fa-out ref_mask-dbtype nuclforidinwtf19 wtf24 wtf4doblastn-query../../outerrepeat_JB22_${id}-dw.fa-db ref_mask-task blastn-outfmt7-out ref_mask.query_${id...
ctjsw@xps:~/blast-lastest$ blastn -query test_query -db test_db -task blastn -dust no -outfmt 7 -num_alignments 2 -num_descriptions 2 ctjsw@xps:~/blast-lastest$ blastn -query test_query -db test_db -task blastn -dust no -outfmt 7 -num_alignments 2 -num_descriptions 2 ``` #...
9.1.3 Mapping a cDNA/EST to a Genome Determining the correct structure of eukaryotic protein-coding genes isnt an easy task because genes are broken up into exons. One of the most accurate methods for determining exon-intron structure involves mapping transcripts back to their origin in a ...