In enzymology, a blasticidin-S deaminase (EC 3.5.4.23) is an enzyme that catalyzes the chemical reaction blasticidin S + H2O rightleftharpoons deaminohydroxyblasticidin S + NH3. Thus, the two substrates of this enzyme are blasticidin S and H2O, whereas its two products are deaminohydroxy...
Resistance to blasticidin is conferred by the blasticidin resistance gene from Bacillus cereus (bsr), which codes for blasticidin-S deaminase. Cell death induced by blasticidin is rapid and cell lines carrying a blasticidin resistance gene can be obtained in less than a week. Typically, mammalian ...
blasticidin S deaminase CAS No.54576-55-5 Chemical Name:blasticidin S deaminase CBNumber:CB21400803 Molecular Formula: Formula Weight:0 MOL File:Mol file Hazard and Precautionary Statements (GHS) Symbol(GHS) Signal word Hazard statements Precautionary statements blasticidin S deaminase Preparation ...
Blasticidin S is a peptidyl nucleoside antibiotic used for the selection of cells expressing the bar gene which encodes for blasticidin deaminase. The antibiotic is active against many bacteria, yeast and animal cells by interfering with the peptide bond formation in the ribosomal machinery of prokaryo...
Blasticidin S用于筛选携带bsr或BSD基因的转染细胞。杀稻瘟菌素S具有快速而强效的作用模式,一个星期内完成阳性克隆的筛选,且很低的抗生素浓度便能导致细胞迅速死亡。 抗性基因: 三种杀稻瘟菌素抗性基因已被克隆且测序:一种是乙酰转移酶基因,来自杀稻瘟菌素产生菌的bls基因;另外两种是脱氨酶基因(deaminase genes),分...
Resistance to blasticidin is conferred by the blasticidin resistance gene from Bacillus cereus (bsr), which codes for blasticidin-S deaminase.Cell death induced by blasticidin is rapid and cell lines carrying a blasticidin resistance gene can be obtained in less than a week. Typically, mammalian ...
Resistance toblasticidinis conferred by theblasticidinresistance gene fromBacillus cereus(bsr), which codes forblasticidin-Sdeaminase. Cell death induced byblasticidinis rapid and cell lines carrying ablasticidinresistance gene can be obtained in less than a week. Typically, mammalian cells are sensitive...
Blasticidin S HCl is a nucleoside antibiotic isolated from Streptomyces griseochromogenes which inhibits protein synthesis in both prokaryotic and eukaryotic cells (Takeuchi et al., 1958; Yamaguchi et al., 1965). Resistance is conferred by expression of either one of two Blasticidin S deaminase gene...
Blasticidin S deaminase gene from Aspergillus terreus (BSD): a new drug resistance gene for transfection of mammalian cells. Biochim. Biophys. Acta. 1219:653-9. Figures Blasticidin Back to the top Specifications Appearance (form): Liquid or powder Appearance (color): Colorless (solution), ...
Blasticidin S用于筛选携带bsr或BSD基因的转染细胞。杀稻瘟菌素S具有快速而强效的作用模式,一个星期内完成阳性克隆的筛选,且很低的抗生素浓度便能导致细胞迅速死亡。 抗性基因: 三种杀稻瘟菌素抗性基因已被克隆且测序:一种是乙酰转移酶基因,来自杀稻瘟菌素产生菌的bls基因;另外两种是脱氨酶基因(deaminase genes),分别...