In Japanese strains, many AMR genes containing blaCTX-M-1and the class 1 integron are highly accumulated in one region on the chromosome, and the AMR of E. coli was enhanced via the IS26-mediated duplication of the AMR gene cluster....
coli isolates was attributed to the carriage of an IncI1 ST-3 plasmid (pCTXM1-MU2) encoding blaCTXM-1. Genomic characterisation of selected E. coli isolates (n=61) identified plasmid movement into multiple commensal E. coli (n=22 STs). Major STs included ST10, ST5440, ST453, ST2514...
IncI1 plasmids are known disseminators of the extended-spectrum cephalosporin resistance (ESC) gene blaCTX-M-1, among species of the Enterobacteriaceae family. In several IncI1 plasmids, this gene was found incorporated into the transposition unit, ISEcp1-blaCTX-M-1-orf477, interrupting a shuf...
Out of 34 isolates resistant to all the cephalosporins used, 41.2% (14) were ESBL-producing, of which blaCTXM-1 and blaCTXM-2 were detected in 85.7%, while blaTEM was seen in 64.3%. Conclusions: blaCTXM and blaTEM may be the predominant ESBL genes haboured in the bacteria pathogens...
鸡源大肠杆菌中耐药基因blaCTX-M的水平转移研究
本研究对四川省规模化鸡场分离出的29株产ESBLs的大肠杆菌进行了blaCTX-M耐药基因检测和接合试验,并对供体菌和得到的接合子进行了耐药表型及基因型比较.结果显示:29株产ESBLs的大肠杆菌中均检出blaCTX-M基因,包括7个亚型;29株大肠杆菌中有11株接合成功,接合效率在在10~(-5)～10~(-3)之间;所有接合子均表现...
The objective of this study was to develop a multiplex PCR (M-PCR) system for the detection of blaSHV, blaTEM, blaCTX-M-1, blaCTX-M-9 and blaOXA-1 group genes and to apply it in clinical Klebsiella pneumoniae and Escherichia coli strains. To do this, we used group-specific PCR ...
目的 探讨产超广谱β-内酰胺酶大肠埃希菌blaC T X-M基因分型及相应耐药特征,以指导临床抗菌药物的合理应用.方法 选取2014年1月到2016年1月医院细菌室分离出的大肠埃希菌69株;对上述菌株进行产超广谱β-内酰胺酶表型及耐药特征的检测;统计产超广谱β-内酰胺酶大肠埃希菌检出率及其基因型的分布;对比不同基因型的...
Infantis pESI-like IncFIB(K)_1_Kpn3 were detected, one associated with the European clone carrying blaCTX-M-1 (21 isolates) and the other associated with U.S. isolates carrying blaCTX-M-65 (2 isolates, pESI-like U.S. variant). The majority was resistant to 3rd generation cephalosporin...