b These competent cell efficiencies are guaranteed when cells are used according to the specifications outlined in this instruction manual. c The pUC18 control plasmid is shipped at a concentration of 0.1 ng/μl in TE buffer (see Preparation of Media and Reagents). STORAGE CONDITIONS Competent ...
BL21 感受态细胞是高水平表达蛋白质且易于诱导的通用菌株。基础 BL21 菌株不含 T7 RNA 聚合酶基因,可与非 T7 RNA 聚合酶蛋白质表达系统配套使用。BL21 最严格地控制毒性极强蛋白质的蛋白质表达。
The cell wall also seems to have greater permeability, rendering the cells more osmosensitive. For best results: Shake ClearColi cells vigorously during growth and vortex the cells for further use (e.g. preparation of serial dilutions) to avoid settling. Make glycero...