BL21-AI One Shot Chemically Competent E. coli 常见问题解答 (FAQ) The BL21-AI strain is suitable for high-level recombinant protein expression from any T7-based expression vector. Because T7 RNA polymerase levels are tightly regulated, the BL21-AI strain is especially useful for the expression...
空运受限产品:发货后, 北上广深及省会城市通常为 2-4天,二线城市 通常3-5天,三线以及偏远地区通常 6-10天,周末以及节假日顺延。 现货中心:此类产品将由您身边的现货中心极速配送。 #BL21 AI ONE SHOT COMP CELLS 规格 Unit Size1 each 文件和下载 ...
关于有毒蛋白的表达,请考虑 BL21-AI™ One Shot™ 化学感受态大肠杆菌。 仅供科研使用。不可用于诊断程序。 规格 产品类型感受态细胞 是否含 F' 附加体缺乏F’附加体 提高质粒质量否 是否可克隆甲基化 DNA是 转化效率级别中等效率 (10^8-10^9 cfu⁄µg) 耐抗生素细菌No 蓝色/白色筛查否 高通量能力不...
For expression of toxic proteins, consider BL21-AI One Shot Chemically Competent E. coli.BL21 Star (DE3) and other strains are available in MultiShot formats for high throughput applications.Explore bacterial growth media formats.We offer a variety of systems for the expression of recombinant ...
关于有毒蛋白的表达,请考虑 BL21-AI™ One Shot™ 化学感受态大肠杆菌。 仅供科研使用。不可用于诊断程序。 规格 产品类型感受态细胞 是否含 F' 附加体缺乏F’附加体 提高质粒质量否 是否可克隆甲基化 DNA是 转化效率级别中等效率 (10^8-10^9 cfu⁄µg) 耐抗生素细菌No 蓝色/白色筛查否 高通量能力不...
关于有毒蛋白的表达,请选择 BL21-AI™ One Shot™ 化学感受态大肠杆菌。如需用于从低拷贝数 T7 启动子表达系统(例如Champion™ pET 载体)中表达非毒性重组蛋白,请选择我们的 One Shot™ BL21 Star™ (DE3) 化学感受态细胞。仅供科研使用。不适用于动物或人类诊断或治疗用途。 仅供科研使用。不可用于诊断...
coli strain, such as BL21-AI cells. - After following the transformation protocol, plate the transformation reaction on LB plates containing 100 µg/mL ampicillin and 0.1% glucose. The presence of glucose represses basal expression of T7 RNA polymerase. - Following transformation of BL21-AI ...
产品规格One Shot 产品线Thermo Scientific™ 数量10 x 100μL Unit SizeEach 内容与储存 • 10 x BL21(DE3) 感受态细胞 (100 μL) • pUC19 DNA (50 μL) (10 pg/μL) • S.O.C.培养基 (5 mL) 在-80°C 下储存。 文件和下载 ...
Try using a tighter regulation system, such as BL21 (DE3) (pLysS) or BL21 (DE3) (pLysE), or BL21(AI). Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center. Once you have obtained your desired construct, we recommend that you store your ...
BL21-AI One Shot Chemically Competent E. coli 常见问题解答 (FAQ) It is imperative that a cloning strain such as TOP10 be used for characterization of the plasmid, propagation, and maintenance. BL21 cells are wild-type for endA and recA, which could result in poor miniprep quality and a ...