Viral load testing for BK Virus (BKV) has become the standard of care for the diagnosis of infection and monitoring of therapy of kidney transplant patients infected with BKV. However, there are currently no FDA-approved BKV quantification assays and no standardization among available tests. This ...
BK病毒检测在骨髓移植中的意义 BK病毒(BK virus,BKV)是肾移植后BKVN的已知病因,但在造血干细胞移植(HSCT)受者中,它通常与出血性膀胱炎(hemorrhagic cystitis,HC)有关。HC发病时可有90% 患者的血和尿 BKV-DNA阳性[1],是HSCT后常见并发症之一。无预防措施情况下发生...
3.免疫组织化学技术 使用SV40病毒T抗原(simian virus 40 T antigen)或BK病毒T抗原(BK-virus T antigen)试剂盒,对石蜡包埋的肾组织进行免疫组织化学染色,如果小管上皮细胞核内着色阳性,即可确诊BKN。 4.电子显微镜技术 在电镜下,细胞核内可见BK病毒的包涵体,为晶体颗粒,直径约40nm,呈致密结晶样排列,根据其大小很容...
Currently recommended BK virus (BKV) plasma viral load cutoff of ≥4 log10/mL underestimates the diag- nosis of BKV-associated nephropathy: a single transplant center experience. Transpl Infect Dis 2014;16(1):55-60.Hassan S, Mittal C, Amer S, Khalid F, Patel A, Delbusto R, et al. ...
Introduction: Polyoma BK virus (BKV) has recently been identified to cause renal allograft dysfunction, which manifests as polyomavirus-associated nephropa... Gary,W C.,Chan,... - 《Renal Failure》 被引量: 2发表: 2012年 Quantification of BK Viral Load in Asymptomatic Renal Allograft Recipients...
Background: Hemorrhagic cystitis (HC) in allogeneic bone marrow transplanted (BMT) patients is associated with BK virus (BKV) reactivation manifested as BK viruria. However, since 77–90% of all adult BMT patients excrete BKV, viral reactivation alone cannot be responsible for HC. Recently, a ...
2.PCR检测技术 BKN的治疗中监测病毒的载量(viral load)是评判疗效的重要指标。PCR检测患者血清BK病毒DNA，病毒超过7700拷贝/ml血清即认为临床阳性标本，该方法诊断BKN的准确率在50以上。Nickeleit等观察在肾功能稳定的患者中定期肾活检，并采用定量 PCR 检测肾组织BK病毒，有助于早期诊断BKN。肾组织聚合酶...
BackgroundBK virus (BKV)-associated nephropathy (BKVAN) is a major cause of renal dysfunction and graft loss in renal transplant recipients. Monitoring plasma BK viral load (BKVL) is the recommended screening tool to predict BKVAN. American Society of Transplantation (AST) guidelines define a B...
Patients who infected with the BK virus were divided into HC group and non-HC group according to occurrence of HC.BK viral load were compared in two groups. Urine BK viral load were analyzed after logarithmic transformation. Data that conforms to a normal distribution is expressed as mean ± ...
Background: BK virus (BKV) is a prominent post-transplant infection for pediatric kidney transplant patients. We do not know what differentiates the patient that manifests BK nephropathy or prolonged BK viral load from the patient who does not. Little is known about genetic changes in the entire...