While identifying threatening new viral genetic material is an important job for our innate immune system, there is typically no reaction against these ancient endogenous benign retrovirus cDNAs that in modern terms are essentially a part of our own genome. Yet scientists have now implicated this evo...
Generally, ramp rates are lowered, and the PCR is run for additional cycles compared to a more standard PCR program. [2] Problems Amplifying GC-rich Regions Summarized So now you understand why amplifying GC-rich regions in your PCR reaction is causing you headaches, but most importantly, you...