Maekawa M,Sugano K,Kashiwabara H,et al.DNA methylation analysis using bisulfite treatment and PCR-single-strand conformation polymorphism in colorectal cancer showing microsatellite instability. Biochemical and Biophysical Research Communications . 1999
PCR analysis is its potential to allow the rapid screening of hundreds to thousands of samples and its sensitivity, making it possible to detect a single methylated allele in 10,000 unmethylated alleles. Unlike other techniques, this assay is completed at the PCR step, without the need for ...
Here we present fC-CET, a bisulfite-free method for whole-genome analysis of 5fC based on selective chemical labeling of 5fC and subsequent C-to-T transition during PCR. Base-resolution 5fC maps showed limited overlap with 5hmC, with 5fC-marked regions more active than 5hmC-marked ones....
2863 Bisulfite genomic sequencing is currently considered the gold standard for DNA methylation analysis, but it is very low throughput, laborious and bias can occur during sub-cloning of bisulfite PCR products. Therefore, we investigate... K Taylor,R Kramer,W Davis,... - 《Cancer Research》 ...
Methylation of the promoter region of ATF5 was assayed by bisulfite-specific polymerase chain reaction (PCR) sequencing analysis in 35 cases of glioma and 5 normal tissues. 收集35临床胶质瘤组织及5例急性脑外伤组织作为对照, 应用亚硫酸盐测序技术检测ATF5的甲基化水平, 并结合临床病理资料进行分析;实时...
Various analyses can be performed on the altered sequence to retrieve this information: bisulfite sequencing, pyrosequencing, methylation-specific PCR, high resolution melting curve analysis, microarray-based approaches, and next-generation sequencing. How it works Treatment of DNA with bisulfite converts...
End-point PCR Using EpiMark® Bisulfite Conversion Kit (E3318) Cycling Protocol Using EpiMark™ Bisulfite Conversion Kit (E3318) 说明书 产品说明书包含产品使用的详细信息、产品配方和质控分析。 manualE3318 工具& 资源 选择指南 DNA Methylation Table FAQs & 问题解决指南 FAQs How shou...
This method combines PCR amplification of the bisulfite-modified DNA with the subcloning of the amplicons into plasmids followed by transformation into bacteria and plating on selective media. The resulting colony forming units are each comprised of bacterial clones containing the same plasmid reflecting...
Interspecific in vitro fertilization, bisulfite sequencing PCR (BSP), RT-PCR, nested RT-PCR, nested real-time qRT-PCR and 2鈻斥柍Ct method, indirect ... FZ Li,LB Li,Y Zhong,... - 《Plos One》 被引量: 21发表: 2012年 Methylation analysis of plasma cell-free DNA for breast cancer ea...
Quantitative PCR analysis: Primer Express (v3.0) Real-Time PCR System Data analysis: Gene ontology (GO) enrichment analysis Methylation-expression correlation analysis Results Researchers investigated the differences between the three cultivars in DNA methylation patterns, DMRs (Differentially methylated...