1. Make 32% Acrylamide/Bis-acrylamide (30:2) solution 30gAcrylamide 2gBis-acrylamide 50ml Milli-Q water Let Acrylamide/Bis-acrylamide dissolve in water, then add water up to 100ml solution. Wrap with aluminum foil, keep at4℃. 2.20×low MW running buffer(1L) MES1000mM195.6g Tris1000mM121...
本预制胶丙烯酰胺(Acrylamide)与甲叉丙烯酰胺(Bisacrylamide)的比例为29:1,凝胶厚度为1.0mm。加样孔数为10孔的最大上样量为50μl,加样孔数为15孔的最大上样量为30μl。胶板尺寸:宽×高×厚度为100×90×4.8mm;凝胶尺寸:宽×高×厚度为84×74×1mm。
本预制胶丙烯酰胺(Acrylamide)与甲叉丙烯酰胺(Bisacrylamide)的比例为29:1,凝胶厚度为1.0mm。加样孔数为10孔的最大上样量为50μl,加样孔数为15孔的最大上样量为30μl。胶板尺寸:宽×高×厚度为100×90×4.8mm;凝胶尺寸:宽×高×厚度为84×74×1mm。
This effect may be intensified for larger proteins, whose migration is more impeded in the low concentration acrylamide of the stacking gel. To alleviate the problem, we recommend concentrating the protein and loading a smaller volume. This gives a "thinner" starting zone. Find addition...
Bolt Bis-Tris Plus gels have a proprietary acrylamide/bisacrylamide formulation that is based on the NuPAGE Bis-Tris gel chemistry but different from it. Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.其他...
本预制胶丙烯酰胺(Acrylamide)与甲叉丙烯酰胺(Bisacrylamide)的比例为29:1,凝胶厚度为1.0mm。加样孔数为10孔的最大上样量为50μl,加样孔数为15孔的最大上样量为30μl。胶板尺寸:宽×高×厚度为100×90×4.8mm;凝胶尺寸:宽×高×厚度为84×74×1mm。 聚丙烯酰胺凝胶电泳(Polyacrylamide gel electrophoresis,...
mPAGE®小型湿法印迹转移系统, for transfer of proteins from a mini acrylamide gel to blotting membrane, accommodates two wet transfer cassettes 文件 分析证书(COA) 输入产品批号来搜索分析证书(COA)。批号可以在产品标签上"批“ (Lot或Batch)字后找到。
AcrylamideGel electrophoresisAsp-Pro bond cleavageNeutral pH gelsThe pH of conventional Tris–glycine SDS–PAGE gels during a run is determined to be 9.5, in contrast to Bis–Tris–Mes gels where the pH is 7.2. Concentrations of free acrylamide are determined to be less than 10 mM in ...
7 M urea, 15% polyacrylamide gel mix 60 mL: 28.8 g urea, 22.5 mL acrylamide:bisacrylamide (19:1), 199.2 μL APS, 24 μL TEMED, 6 mL 10 × TBE (add APS and TEMED immediately before pouring gel) (see tip 1 and 2) • 10 × reaction buffer: 100 mM Bis-Tris-Propane-HCl (pH...
TBE电泳缓冲液12Tris碱54g硼酸27EDTApH5MEDTA溶液pH称取1861g的EDTANa2•2H2O并溶于水中加NaOH调节pH至8Westernblot实验溶液配制5ml1MTrisHcl125ul3MNacl125ul10SDS25ulTriton10025uldH2OArcbis溶液30Acrylamide29Bisacrylamide溶于超纯水定容至100ml过滤30Arcbis665mlTrispH值895ml10SDS25ml10APS25mlTemed15ml加dH2O...