1. Make 32% Acrylamide/Bis-acrylamide (30:2) solution 30gAcrylamide 2gBis-acrylamide 50ml Milli-Q water Let Acrylamide/Bis-acrylamide dissolve in water, then add water up to 100ml solution. Wrap with aluminum foil, keep at4℃. 2.20×low MW running buffer(1L) MES1000mM195.6g Tris1000mM121...
本预制胶丙烯酰胺(Acrylamide)与甲叉丙烯酰胺(Bisacrylamide)的比例为29:1,凝胶厚度为1.0mm。加样孔数为10孔的最大上样量为50μl,加样孔数为15孔的最大上样量为30μl。胶板尺寸:宽×高×厚度为100×90×4.8mm;凝胶尺寸:宽×高×厚度为84×74×1mm。
本预制胶丙烯酰胺(Acrylamide)与甲叉丙烯酰胺(Bisacrylamide)的比例为29:1,凝胶厚度为1.0mm。加样孔数为10孔的最大上样量为50μl,加样孔数为15孔的最大上样量为30μl。胶板尺寸:宽×高×厚度为100×90×4.8mm;凝胶尺寸:宽×高×厚度为84×74×1mm。
本预制胶丙烯酰胺(Acrylamide)与甲叉丙烯酰胺(Bisacrylamide)的比例为29:1,凝胶厚度为1.0mm。加样孔数为10孔的最大上样量为50μl,加样孔数为15孔的最大上样量为30μl。胶板尺寸:宽×高×厚度为100×90×4.8mm;凝胶尺寸:宽×高×厚度为84×74×1mm。
本预制胶丙烯酰胺(Acrylamide)与甲叉丙烯酰胺(Bisacrylamide)的比例为29:1,凝胶厚度为1.0mm。加样孔数为10孔的最大上样量为50μl,加样孔数为15孔的最大上样量为30μl。胶板尺寸:宽×高×厚度为100×90×4.8mm;凝胶尺寸:宽×高×厚度为84×74×1mm。
This effect may be intensified for larger proteins, whose migration is more impeded in the low concentration acrylamide of the stacking gel. To alleviate the problem, we recommend concentrating the protein and loading a smaller volume. This gives a "thinner" starting zone. Find additional tips, ...
Bolt Bis-Tris Plus gels have a proprietary acrylamide/bisacrylamide formulation that is based on the NuPAGE Bis-Tris gel chemistry but different from it. Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center. Our precast protein...
Acrylamide-Bis-Acrylamide Solution(丙烯酰胺-甲叉双丙烯酰胺溶液),30%,39:1 Carbonate Buffer(碳酸盐缓冲液),0.5M,pH9.6 MOPSO Buffer(MOPSO溶液),0.2M,pH6.0 RNase-free 75% Ethanol(无RNase的75%乙醇) Zymogramm Sample Buffer(酶谱样品缓冲液) TB Solution-IV(TB溶液Ⅳ) 无RNase的DNase溶液 Casein-Tween...
Pre-mixed acrylamide buffer solutions save time and improve reproducibility Flexibility: dilute resolving solution with DI water to any gel percentage between 8% and 15% Quick Cast protocol allows simultaneous polymerization of stacking and resolving gels, saving even more time Application Premixed solutio...
展开 MGT mPAGE® Mini Gel Tank, for electrophoresis of precast gels or hand cast polyacrylamide gels 展开 MWTS mPAGE®小型湿法印迹转移系统, for transfer of proteins from a mini acrylamide gel to blotting membrane, accommodates two wet transfer cassettes 展开文件...