Two major classes of hydroxy fatty acids are formed by hydroxylation of the 蠅-side chain and by hydroxylation of bisallylic methylene carbons. Bisallylic cytochrome P450-hydroxylases transform linoleic acid to 11-hydroxylinoleic acid, arachidonic acid to 13-hydroxyeicosa-5Z,8Z,11Z,14Z-tetraenoic...
Polyunsaturated fatty acids can be oxygenated by cytochrome P450 to hydroxy and epoxy fatty acids. Two major classes of hydroxy fatty acids are formed by hydroxylation of the omega-side chain and by hydroxylation of bisallylic methylene carbons. Bisallylic cytochrome P450-hydroxylases transform linoleic...
Hydroxylations of carbons at the ω end and epoxidations of the double bonds have attracted greatest attention. ω-Hydroxylation of polyunsaturated fatty acids is efficiently catalyzed by CYP4A, while other enzymes catalyze ω2 and ω3 hydroxylations of arachidonic acid (20: 4n-6). Enzymes of ...
Microsomes from beta-naphthoflavone-treated rats hydroxylated linoleic acid without position specificity, i.e., at carbons 8, 11, 14, 16, 17, and 18. 11-HODE, 17-HODE, and 18-HODE were major metabolites. Microsomes from rats treated with phenobarbital, isopropanol, imidazole, or acetone ...