After the first transformation in the cultivation media, washed cells were used again for transformation, and a considerable amount of galactitol was accumulated in the reaction mixture. This study revealed that after lactose is split by 尾- d -galactosidase, whey can be used directly without ...
Specifically, a yeast strain capable of consuming lactose intracellularly is engineered to produce tagatose from lactose through three layers of manipulations. First, GAL1 coding for galactose kinase is deleted to eliminate galactose utilization. Second, heterologous xylose reductase (XR) and galactitol ...
molecules Article Immobilized Trienzymatic System with Enhanced Stabilization for the Biotransformation of Lactose Pedro Torres and Francisco Batista-Viera * Cátedra de Bioquímica, Departamento de Biociencias, Facultad de Química, Universidad de la República, Gral Flores 2124, 11800 Montevideo, Uruguay...