Furthermore, side effects of excess biotin include increased acne, as well as increased risk of miscarriage, throat swelling, nausea, itchy rashes, facial swelling, and chest tightness or pain. Biotin supplements may also interact negatively with anti-seizure and diabetes medications, as well as dr...
There are no know Biotin side effects; however there are risks of biotin deficiency. On the plus side, Biotin has many health benefits - it's used to treat hair loss and a number of skin disorders. Vitamin B7 Biotin RDA In 1998 the Food and Nutrition Board of the Institute of Medicine...
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The purified protein was dialyzed against PBS (135 mM NaCl, 2.7 mM KCl, 4.3 mM Na2 HPO4, 1.4 mM KH2 PO4) buffer, and antiserum was obtained by immunizing a female New Zealand White rabbit. Immunoblot analysis. For immunoblot analysis, proteins were extracted as follows: Alfalfa leaf ...
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