Baez-Saldana A, et al.: Biotin deficiency and biotin excess: Effects on the female reproductive system. Steroids 2009;74:863-869.Baez-Saldana A, Camacho-Arroyo I, Espinosa-Aguirre JJ, Neri-Gomez T, Rojas-Ochoa A, Guerra-Araiza C, Larrieta E, Vital P, Diaz G, Chavira R, Fernandez-...
An intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines
Human biotin deficiency is rare because intestinal bacteria can produce biotin in excess of the body's daily requirements. However, several conditions can cause a deficiency, such as exposure to the binding protein, avidin, and metabolic disorders, such as a deficiency in the carboxylase synthetase...
Another thing to note here is that since the excess biotin is excreted out of the body, there really is no fear of a biotin over dose. Many health institutions don’t have a set dosage for biotin for that precise reason. Also there are no side effects to excess biotin. It will get m...
(2µL or 6-7µg protein per reaction). In the case of the NF-κB system, nuclear extracts were made from HeLa cells that had been induced with TNFα or cells that were untreated. Competition reactions containing a 200-fold molar excess of unlabeled duplex were performed to illustrate ...
4. Add sufficient volume of the concentrated Sulfo-NHS-LC-Biotin to give a 12-fold molar excess of biotin to a 10 mg/ml IgG solution or a 20-fold molar excess of biotin to a 2 mg/ml IgG solution (See Table 1 for examples). 5. Place the reaction mixture on ice and incu- bate ...
Given the chatter that this is a gold-standard ingredient for hair growth, you may be super tempted to hit "add to cart" on the first product you see if you've noticed changes to your hair such as dryness, brittleness, and excess breakage. But is biotin in all of its forms really wo...
(BCCP) for protein biotinylation. On the other hand, it binds to thebioOsequence of biotin operon to inhibit the expression of biotin synthesis genes (Fig.3) [92]. In this way, it ensures that when the biotin is in excess for normal cell growth, cells halt biotin synthesis to alleviate...
?1.Calculate millimoles of biotin reagent to add to the reaction for a 20-fold molar excess:?mL protein × (mg protein/mL protein) × (mmoL protein/mg protein) × (20 mmoL protein/mmoL protein) = mmoL Biotin.?* 20 = Recommended molar fold excess of biotin for 2 mg/ml protein ...
Only use the appropriate amount of shampoo when washing your hair. Using too much shampoo can have negative effects on your hair. Rinsing excess shampoo is stressful. If not washed out well, it might leave white flakes on your hair.