Buffer BX; Biotin Elution Buffer - Get the Best Quote/Price and read Reviews, Features and Research Applications
产品名称:Buffer BE; Biotin Elution Buffer; 10 x concentrated .0订购此产品 供应商:IBA 规格:25 ml 目录价:914.628 库存状态: CAS编号: 应用范围: 种属来源: 相关信息: 保存条件: 说明书地址:点击查看详细 打印此页关闭此页 上一个:Strep-tag II peptide[2-1018-009/5 x 1.8 mg] ...
The elution buffer disrupts the interaction between the lig- and and its binding partner by pH extremes (low or high), high salt, presence of detergents, chaotropic agents that unfold one or both of the molecules, or the removal of some factor required for the pair to bind. Once released,...
Elution method Elution with acid or SDS-PAGE loading buffer for single-use applications Application Purification of (strept)avidin-coupled proteins and nucleic acids, IP, Co-IP, DNA-protein pulldowns 本产品为50%凝胶悬液,包装体积为总体积,每毫升本产品中共含有0.5ml凝胶沉淀物。如果每个样品使用50μl...
The elutions were analyzed by western blot. Percentages beneath target indicate elution efficiency compared to bead-boil. Continue reading: Continue reading: Overview of the Immunoprecipitation (IP) Technique Continue reading: Immunoprecipitation ...
Elution methodElution with acid or SDS‐PAGE loading buffer for single-use applications ApplicationPurification of (strept)avidin-coupled proteins and nucleic acids, IP, Co-IP, DNA-protein pulldowns 如果每个用品使用20μl磁珠,每毫升本产品可以用于50个样品。
Strep-tag II Desthiobiotin elution reagent 2-1000-025 25 ml Strep-tag II Peptide 2-1018-002 1.8 mg Strep-tag regeneration buffer with HABA 2-1002-100 100 ml Strep-tag washing buffer 2-1003-100 100 ml WET FRED For 1 ml Gravity flow columns 2-0911-001 1 Set WET FRED For 5-10 ml ...
(i) denaturation of streptavidin by boiling the resin in a denaturing buffer that may include high concentrations of chaotropic salts, (ii) trypsin digestion of proteins while they are bound to the resin, or (iii) elution of proteins with excess free biotin. These protocols can co-elute ...
Excess DTT was removed by molecular sieve chromatography using 50mM Tris, pH 8.3, with 5mM EDTA as the elution buffer. The IgG with reduced sulfhydryl groups was then biotinylated; 30 μl of 4mM iodoacetyl-LC-biotin in Me2SO was added and the mixture was incubated for 90min at room ...
Elution Buffer: 50 mM sodium acetate, pH 4.0 3. Purification Procedure This procedure is optimized for a column of 0.5 ml bed volume. The volumes of the reagents can be scaled up or down according to the size of the column. Sample Preparation ...