Biotin biosynthesis was investigated in lavender cell cultures ( Lavandula vera L.). Two different biological assays and two different HPLC procedures were used to identify all the intermediates involved in biotin biosynthesis. The pathway for biotin biosynthesis could be analyzed starting with [ 3 H...
Baldet P, Gerbling H, Axiotis S, Douce R (1993b) Biotin biosynthesis in higher plant cells. Identification of intermediates. Eur J Biochem 217:479–485Baldet, P., Gerbling, H., Axiotis, S., and Douce, R. (1993). Biotin biosynthesis in higher plant cells. Identification of ...
The inhibition was restored by the intermediates of biotin biosynthesis, dethiobiotin (DTB) and 7, 8-diaminopelargonic acid (DAPA), as well as biotin. However, the intermediates in earlier steps, 7-keto-8-aminopelargonic acid (KAPA) and pimelic acid, had no recovering effect. When Rhodotorula...
The penultimate step in biotin biosynthesis is catalyzed by dethiobiotin (DTB) synthase BioD, which converts DAPA into DTB [68]. This reaction involves the introduction of a carbon atom between the N7 and N8 nitrogen atoms of DAPA to form a ureido ring [68]. The catalytic process involves ...
The genetics and mechanistic enzymology of biotin biosynthesis has been the subject of a reinforced activity in the last two decades. Four enzymes are involved in the synthesis of biotin from its two precursors, alanine and pimeloyl CoA. They are now well-characterized and their X-ray structures...
Biotin biosynthesis Biotin biosynthesisBiotin biosynthesisFirst pagedoi:10.1351/pac199365061249MarquetA.De GruyterPure & Applied ChemistryAndree Marquet.Biotin biosynthesis.Pure and Applied Chemistry. 1993Biotin biosynthesis. Marquet,A. Pure and Applied Chemistry . 1993...
Regulation of biotin biosynthesis During microbial growth, cells can obtain biotin through two mechanisms: uptake from the environment or de novo synthesis [85]. Typically, the genes involved in biotin synthesis are organized in an operon, ensuring simultaneous expression for biotin production [86]. ...
It was found that pimelic acid was essential substrate in biosynthesis of biotin-vitamers and that some amino acids and organic acids stimulated the biosynthesis of biotin-vitamers from pimelic acid. Alanine was found to be most effective. It was assumed that, in the presence of pimelic acid,...
However, biotin biosynthesis has been overlooked for priority pathogens such as Acinetobacter baumannii, Klebsiella pneumoniae and Pseudomonas aeruginosa. This can be attributed to the lack of attenuation observed for biotin biosynthesis genes during transposon mutagenesis studies in mouse infection models6,7...
2002. Total synthesis of amiclenomycin, an inhibitor of biotin biosynthesis. Chem Eur J 8:439- 450.S. Mann, S. Carillon, O. Breyne, A. Marquet, Total synthesis of amiclenomycin, an inhibitor of biotin biosynthesis, Chemistry, 8 (2002) 439-450....