We will then move on to different classes of cells that are grown in culture: primary cells, cancer cells, and cell lines. Finally, we will discuss main components of cell media by looking at what is most commonly used for mammalian cell cultures....
Second, we have to separate correct and incorrect annotations, as is the case for library search or peptide annotation in shotgun proteomics; this allows one to concentrate downstream analysis on novel compounds most likely to be correctly annotated. Naturally, one might want to use the hit ...
Although spatial transcriptomics (ST) has become a powerful tool for delineating spatial gene expression in primary tissue specimens, commonly used platforms, such as 10x Visium, remain limited to bulk gene expression measurements, where each spatially resolved expression profile is derived from as many...
Morpho-Agronomic Performances and Antioxidant Properties in Chilli Pepper Hybrids are highlighted by Multi-Scale Evaluation on Two Locations and Digital Fruit Imaging Author(s):Miguel Jose Chilli peppers are a vegetable crop that is commonly eaten fresh or dried as a spice. The nutritional value deri...
At present, transcriptomics is the most commonly used method for identifying candidate genes based on gene expression levels. However, at the genomic level, genome-wide association studies (GWAS) are frequently used to identify major genes or genetic markers [8,9,10,11]. For instance, a ...
With the increasing knowledge about nanotechnology, especially related to nanoparticles, nanostructures, and, more specifically, the preparation of scaffolds, electrospinning has become the most frequently used technique. This technique is preferred over solvent casting and phase-separation because the ...
tubingensis that are used in the industry. In some cases, the latter have been misidentified as A. niger, and A. niger is by far most commonly used species in the industry (Frisvad et al. 2011). A. luchuensis is found in fermented Puerh tea (Mogensen et al. 2009) and is used ...
importantgenesinrice. 人类iPS细胞的记忆 已有研究表明小鼠诱导性多功能干细胞(iPSCs)保留着它们起源的细胞类型的表观遗传“记忆”。 Kim等对人体细胞进行了研究,比较了重编程过程中DNA甲基化的不完全擦除和异常的重新甲基化。 结果表明:人胚胎千细胞与诱导性多功能干细胞在蛋白质组和磷酸蛋白质组上存在差别,不同...
productionstogetherwiththeprocessthataremostcommonlyused.Finally,anoverviewofthedifferenttypes ofproteinsthathavebeenproducedinthatworkhorseispresented. Keywords:Pichiapastoris,recombinantprotein,bioreactor,glycoengineering INTRODUCTION Yeastsaresuitablehostorganismsfortheproductionof recombinantproteinssincetheycombineeaseof ge...
The reliance on a solitary linear reference genome has imposed a significant constraint on our comprehensive understanding of genetic variation in animals. This constraint is particularly pronounced for non-reference sequences (NRSs), which have not been