Coenzyme B12CorrinVitamin B 12 (cyanocobalamin) is the normal isolated form of coenzyme B 12 (adenosylcobalamin), a structure of marvellous architecture and amazing biological activity. It belongs to the family of tetrapyrroles which includes inter alia the haems and the chlorophylls. This review ...
The bacterium Salmonella typhimurium is capable of a complete synthesis of B12, but does so only under anaerobic conditions. Since sophisticated genetics can be done in this bacterium, we propose a genetic approach to understanding the biosynthesis of vitamin B12 in this organism. We will identify ...
Biosynthesis of vitamin B12 in anaerobic bacteria.Experiments on the incorporation of erythrose and formate into the 5,6-dimethylbenzimidazole moiety of vitamin B12 are described. In one experiment, a 1:1 mixture of D-[1-13C]erythrose and D-[1-13C]threose was added to a Eubacterium limo...
Biosynthesis of vitamin B12: stepwise amidation of carboxyl groups b, d, e, and g of cobyrinic acid a,c-diamide is catalyzed by one enzyme in Pseudomonas d... The cobalamin biosynthetic pathway enzyme that catalyzes amidation of 5'-deoxy-5'-adenosyl-cobyrinic acid a,c-diamide was purified...
vitamin B12CbiFcobalt‐precorrin‐4Bacillus megateriumS‐adenosyl‐ L‐methionineThe Bacillus megaterium cbiF, encoding the cobalt-precorrin-4 S-adenosyl- L-methionine-dependent transmethylase of the anaerobic cobalamin biosynthetic pathway, has been cloned and overexpressed as a His-tagged recombinant ...
The only known source of vitamin B12 (adenosylcobalamin) is from bacteria and archaea. Here, using genetic and metabolic engineering, we generate an Escherichia coli strain that produces vitamin B12 via an engineered de novo aerobic biosynthetic pathway.
Biosynthesis of Vitamin B12 Radioactivity from [1鈥 - 14 C]riboflavin was incorporated into the 5,6-dimethylbenzimidazole moiety of Vitamin B 12 in the aerobes Bacillus megaterium, No... Vera Hllriegl,L Lamm,J Rowold,... - 《Archives of Microbiology》 被引量: 122发表: 1982年 Riboflavin ...
Transport of Vitamin B12 in Escherichia coli: Common Receptor Sites for Vitamin B12 and the E Colicins on the Outer Membrane of the Cell Envelope The first step in the transport of cyanocobalamin (CN-B(12)) by cells of Escherichia coli was shown previously to consist of binding of the B(...
Vitamin B12: how the problem of its biosynthesis was solved - Blanche, Cameron, et al. - 1995 () Citation Context ...is of the corrin ring have been described on the basis of the timing of cobalt insertion into the ring. The late cobalt insertion or aerobic pathway has been well ...
摘要: PD18 STUDIES ON THE BIOSYNTHESIS OF VITAMIN B12 AND CHLOROPHYLL-A Kajiwara Masahiro , Kurumaya Katsuyuki , Okazaki Takeo International Symposium on the Chemistry of Natural Products 1988, 570, 1988-05-29 DOI: 10.24496/intnaturalprod.1988.0_570 年份: 1988 收藏...