The luciferase transgene can be genetically inserted at the first intron of a target gene to act as a surrogate for the gene's endogenous expression in cells and transgenic mice. Alternatively, promoter sequences can be excised and/or amplified from genomic sources or constructed de novo and ...
Based on a large dataset and extensive knowledge from the literature, we show that the ability to bioluminesce may be strongly driven by habitat. We hypothesize that this is probably related to differences in optical properties between pelagic and benthic environments (Fig. 4). While more than ...
Applied to cross-cell activation, bioluminescence can potentially be used as a universal life-life communication strategy where information is carried by photons, creating a species-agnostic communication interface, as envisioned in the movie Avatar [79]. For such communication, an optical synapse can...
2004). The amount of luciferase producedin vivocan be quantified by photons as a measure of promoter activity. Bioluminescent imaging offers the advantage of identifying mice with tumors and specifically measuring tumor cell response to treatment non-invasively, allowing each mouse to be its own ...
Fishes control the output of light intensity using different mechanisms which can be categorized as being under control of hormonal or neuronal pathways. Usually, neural control allows fast and direct response to the photocytes while endocrinal systems allow to control the light produced by bacteria...
A. Bioluminescent creature uses a substance called luciferin and an enzyme to produce light in a biologically driven chemical reaction. B. Bioluminescence can be used to achieve various and sometimes seemingly opposite goals such as frightening away predators or luring prey. ...
When this fluctuation is averaged over several cycles in order to obtain an approximated timing of time series increase, a phase can be computed via waveform analysis. A waveform analysis72 was carried out on biological and floor orientation data, by subdividing time series on the length of the...
Amyloidogenic processing is driven by β-secretase, which cleaves APP at the N-terminal site of the Aβ domain. Both α- and β-secretase cleavages produce C-terminal stubs (termed C83 and C99, respectively) that remain inserted in the membrane and are either degraded or further processed ...
the light output by an actual sample is very low, and the monitor has to be calibrated at this low level where even individual photons can be detected. Thus, the output of the LED has be to substantially attenuated, for example by placing a large block of semi-opaque plastics material bet...
Bioluminescenceimaging is based on the conversion ofluciferinsinto light by theenzymeluciferasefrom insects, such as the North American firefly, and sea species.12,13Bioluminescence imagingcan be used for in vivo detection of various processes in living animals, upon transgene generation expressing lucife...