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The percentage of cells expressing CDH1 and/or TWIST1 in the epicardial layer of treated and control epicardioids (day 15) was calculated (k). Data are presented as means ± s.d. and were analyzed by unpaired two-tailed t-test; *P = 0.03; **P = 0.002; n = 9 ...
Imaging was performed using an inverted Nikon Eclipse Ti-E microscope with perfect focus system, an oil immersion objective (Nikon Plan Apo λ 100× NA 1.45), using two EMCCD cameras (Photometrics Evolve 512), which are mounted on a spinning disc unit (CSU-W1, Yokogawa). TIRF illumination ...
Biology exam. That’s why we’ve selected these 500 AP-style questions and answers that cover all topics found on this exam. The targeted questions will prepare you for what you’ll see on test day, help you study more effectively, and use your review time wisely to achieve your best ...
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Image acquisition before, and after, cell ablation was at 10 s intervals with a Plan-Neofluor 40×/1.3 (numerical aperture) oil immersion objective from an inverted microscope with laser scanning unit LSM 780 (Zeiss), coupled with a two-photon femtosecond laser (Chameleon-XR Ti-Sapphire; ...
The fundamental operative unit of a cancer is the genetically and epigenetically innovative single cell. Whether proliferating or quiescent, in the primary tumour mass or disseminated elsewhere, single cells govern the parameters that dictate all facets of the biology of cancer. Thus, single-cell anal...
Finally, RNA-seq can be coupled with different types of biochemical assay to analyze many other aspects of RNA biology, such as RNA–protein binding, RNA structure, or RNA–RNA interactions. These applications are, however, beyond the scope of this review as we focus on ‘typical’ RNA-seq...