Download our Biology-inspired Google Slides themes and PowerPoint templates and create presentations that feel alive ✓ Free ✓ Easy to edit ✓ Professional
In recent years, research has progressively increased the importance of considering sex differences in stress and fear memory studies. Many studies have traditionally focused on male subjects, potentially overlooking critical differences with females. Em
The mKate2 coding sequence fused to an HA tag was amplified by PCR from the p3E-mKate2-HA no-pA plasmid (Addgene, 80810) as a template and inserted into SwaI–PacI sites on the pAAVS1-CAG-FRT-flanked STOP-poly(A) plasmid. Primers used for cloning and sequencing of the pAAVS1-...
Total RNA was converted into double-stranded cDNA libraries as a template for high-throughput sequencing using the SMARTer stranded total RNA-seq kit v.2–pico input mammalian kit (TaKaRa Bio) according to the manufacturer’s instructions. Briefly, after first-strand cDNA synthesis with SMARTer ...
The important role of unconventional myosin VI (MVI) in skeletal and cardiac muscle has been recently postulated (Karolczak et al. in Histochem Cell Biol 1
The arbuscular mycorrhizal (AM) symbiosis consists of a mutualistic relationship between soil fungi and roots of most plant species. This association provides the arbuscular mycorrhizal fungus with sugars while the fungus improves the uptake of water and
Article CAS PubMed Google Scholar Novosad JA, Kalantaridou SN, Tong ZB, Nelson LM: Ovarian antibodies as detected by indirect Immunofluorescence are unreliable in the diagnosis of autoimmune premature ovarian failure: a controlled evaluation. BMC Womens Health. 2003, 3 (1): 2-10.1186/1472-6874...
HEK and HeLa cells. Normalization was performed as described in Additional file5. (b) Verification of underrepresentation ofCYLDandSOATin HEK and HeLa cells by Real-Time PCR using the same mRNA as template. Expression of genes was normalized to the median of 2 housekeeping genes (PGK,DCTN2)....
Long Expand Template PCR System kit (Roche Diagnostics, Inc.) was used in a 50 μl final volume master mix consisting of final concentrations of 1× PCR buffer 3, dNTPs 0.2 mM, 0.25 mM Lac Z primers, Taq Pol Mix 0.75 u. Forty eight μl from this volume were aliquoted into each ...
Roots are an attractive system for genomic and post-genomic studies of NaCl responses, due to their primary importance to agriculture, and because of their relative structural and biochemical simplicity. Excellent genomic resources have been established