All fragments were spotted onto a 20 × 20-cm Nytran SuPerCharge nylon membrane (Schleicher and Schuell, Keene, NH, USA) using a BioGrid spotting robot (BioRo- botics) in a 4 × 4 grid organization. Each gene was spotted twice in two different membrane grids corresponding to four ...
Assay-ready plates were generated at the UW-Madison Small Molecule Screening Facility with a Labcyte Echo 650 (Beckman Coulter, Brea, CA) liquid handler by spotting each compound onto 96-well assay plates (Greiner Bio-One, Frickenhausen, Germany), which were sealed with adhesive foil, wrapped...
12 bases (four Black-eyed white coat color amino acids) located two amino acids Anemic due to lack of erythrocytes downstream from tyrosine autophosphorylation < 1% of MC number of wild type site of CD117 (Ws, White Spotting mutation) Lack of Fc RI -subunit expression Restoring MCs after ...
Schaller CW, Qualset CO (1975) Isogenic analysis of productivity in Barley: Interaction of genes affecting awn length and leaf-spotting1. Crop Sci 15:378–382 Google Scholar Spreitzer RJ, Salvucci ME (2002) Rubisco: structure, regulatory interactions, and possibilities for a better enzyme. Ann...
So the lecturer said suppose you had a test that was 99% accurate in spotting a rare disease, say affecting 1 person in 1,000. This meant that giving the test to 1,000 people would say that 10 people in that group had the disease, when probably only 1 did. For any probability ...
Adjusted extracts were further diluted with CLB1 buffer to a final spotting concentration of 0.1 μg/μl. Diluted extracts were printed at four serial dilutions (1.6-fold) onto Zeptosens hydrophobic chips (Bayer Technology Services GmbH) using a microarray printer (NanoPlotter 2.1, GeSiM, ...
PF carried out subtracted cDNA libraries, DNA sequencing, DNA amplification for cDNA spotting, hybridization probes and participated in data analysis and manuscript preparation; JDR directed data microarray and statistical analysis, LF participated in quantitative real time reactions, HEH coordinated the wor...
The product of KIT is likely involved in the white spotting of the coat, not only in cattle but also in other domesticated mammals [64]. Our findings are consistent with the observation that while the coat color of Ankole is predominantly red, it is also sometimes white-spotted [61]. ...
Lateral root formation also involves genes from the LATERAL ORGAN BOUNDARIES (LOB) family; consistent with this requirement, we observed that the class I sub-group of this family was exclusively enriched in up-regulated transcripts, with at least seven genes induced following NaCl-treatment (Table ...
Adjusted extracts were further diluted with CLB1 buffer to a final spotting concentration of 0.1 μg/μl. Diluted extracts were printed at four serial dilutions (1.6-fold) onto Zeptosens hydrophobic chips (Bayer Technology Services GmbH) using a microarray printer (NanoPlotter 2.1, GeSiM, Gross...