Animals were kept in a net cage (width: 60 cm, length: 50 cm, height: 90 cm) at room temperature (~ 23 °C), with a humidity 40% and 14:10 h of light–dark cycle; 10% honey solution was prepared from commercially available “Bee easy wild flower honey” (Langnese, Germany) ...
The solution NMR structures of the eight designs have been deposited in the Protein Data Bank under accession numbers 7BPL (NF1-14), 7BPM (NF2-02), 7BQE (NF3-03), 7BQC (NF4-04), 7BPP (NF5-03), 7BQB (NF6-02), 7BPN (NF7-04) and 7BQD (NF8-01). The NMR data have been...
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The concentration of the resulting 1.5-ml solution was determined to be 0.20 ± 0.03 mM by a BCA assay (Pierce, ThermoFisher Scientific); and this solution was concentrated to 0.50 ml (0.60 mM). A 200-µl aliquot (0.12 µmol) of this solution was diluted to 294...
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On silk fiber assembly, the Gly/Ala rich regions (thought to be helical in solution) form a β sheet rich structure in which, unlike amyloids, the strands run parallel to the fiber axis, whereas the Gly/Pro/Gln segments adopt an uncharacterized structure that gives the elasticity to the ...
A puzzling feature about the World Bank (and indeed most international organizations) is the question of who is in charge, who has power. This chapter examines the principal actors that shape the governance of the Bank, and focuses on tw... D Kapur,IJ Pincus,Jeffrey Winters 被引量: 11发表...
FlA is a hexamer in solution and trimer in crystal, and three SAM molecules are bound by a trimer, between the N-terminal domain of one subunit and the C-terminal domain of the adjoining subunit. This arrangement, however, appears to be dependent on a long (24 amino acids) loop in the...
Divide the flowers into two groups, and while wearing eye protection and gloves, spray one group with a solution of either putrescine or cadaverine. (Putrescine dihydrochloride is typically available in 98 percent concentration; this can be diluted to approximately 50 percent for this experiment.) ...
Currently, a refined MR solution is typically used as the starting point for some combination of automatic and manual building of missing portions into the density. In many cases, however, it appears that AF2 predictions will support a more ‘top-down’ approach, in which all residues predicted...