Combining cryopreservation of germline stem cells (GSCs) with their subsequent transplantation into recipient fish is a powerful tool for long-term preservation of genetic resources of endangered fishes. However, application of this technique has been li
2023a). m6A dot plot experiment Mix the cellular RNA and the organism RNA with an equal volume of 20 × SSC buffer (S6639, Sigma-Aldrich, USA). Incubate the mixture at 65 °C for 5 min to induce denaturation. Next, add 100 ng, 200 ng, or 400 ng of poly(A) + RNA ...