This is presently not practical, and methods for more detailed and focussed analysis of smaller numbers of (e.g., FACS isolated) cells are required. In the present case, we use this approach to annotate cell populations with isoform information and to inform single-cell resolution analysis by ...
they face limitations in detailing the transient kinetics that lead to these endpoints. The capabilities of MaBoss and SQUAD to simulate temporal evolution offer practical solutions
More specifically, the inhibition of IL-1Ra in β-glucan-trained WT mice partially reduced the expansion of LKS, LTHSC, LKS+CD150+CD48+ cells, and MPPs. Next, we assessed the impact of impaired IL-1 signaling on the β-glucan-induced protective effects against Mtb in vivo (Figure 5D)....
For further annotation, a set of genes unnamed by the automated pipeline were manually annotated. As part of an ongoing project to investigate hemogenic endothelium commitment and HSC production [14], transcriptomes were produced for two cultured cell fractions. Study of these cells is critical for...
Benjamini Y, Hochberg Y: Controlling the false discovery rate: a practical and powerful approach to multiple testing. JR Statist Soc B. 1995, 57: 289-300. Google Scholar Rhee SH, Hewitt JK, Young SE, Corley RP, Crowley TJ, Stallings MC: Genetic and environmental influences on substance in...
From these case studies, we have drawn a few lessons on the practical challenges that may arise in applying topic modeling approaches to single-cell data, and we share these lessons here (see also [47, 49] for related discussion). One practical question is how to choose K, the number of...
Transcripts encoding mitochondrial HSPs are particularly involved in mitochondrial dysfunctions as part of retrograde signals [107]. Two MitochondrialHSPs,HSP60, andmtHsc70–1are found among the differentially expressed genes up-regulated inpoco1. It was previously suggested that many of NGEMPs such as...
the limiting factor for the integrity (and consequently, the activity) of the node. Of course, the scenarios for the nodes are idealizations of a reality which may be much more complex, but they have demonstrated to represent a realistic enough scenario and to work in practical terms [95]....
(briefly described below). For practical and historical reasons, the model systems have been traditionally terrestrial animals [36], and it is for this particular (biased) reason that we start our description of what is known on niches by analyzing these terrestrial models. They should serve us...
the role of viral infections in directly infecting blastocyst and trophoblast and inducing chronic and uncontrolled inflammation and disrupting microRNAs expression can make this review strongly attractive and practical. We aim to provide an overview of viral infections as the potential etiology of unsucces...