This is presently not practical, and methods for more detailed and focussed analysis of smaller numbers of (e.g., FACS isolated) cells are required. In the present case, we use this approach to annotate cell populations with isoform information and to inform single-cell resolution analysis by ...
Beresford JN, Owen ME (1998) Handbooks in practical animal cell biology: marrow stromal cell culture. United Kingdom: Cambridge University Press Google Scholar 42 F.L. Quito, J. Beh, O. Bashayan, C. Basilico, R.S. Basach Effects of fibroblast growth factor-4 (k-FGF) on long-term cul...
From these case studies, we have drawn a few lessons on the practical challenges that may arise in applying topic modeling approaches to single-cell data, and we share these lessons here (see also [47, 49] for related discussion). One practical question is how to choose K, the number of...
Several approaches have been used to further characterize the molecular regulators of HSCs (e.g., ex vivo screening for factors supporting HSC maintenance), and the identification of these factors has made it feasible to classify specific cell types involved in the various activities carried out wit...
More specifically, the inhibition of IL-1Ra in β-glucan-trained WT mice partially reduced the expansion of LKS, LTHSC, LKS+CD150+CD48+ cells, and MPPs. Next, we assessed the impact of impaired IL-1 signaling on the β-glucan-induced protective effects against Mtb in vivo (Figure 5D)....
(briefly described below). For practical and historical reasons, the model systems have been traditionally terrestrial animals [36], and it is for this particular (biased) reason that we start our description of what is known on niches by analyzing these terrestrial models. They should serve us...
and modelers frequently resort to calibrating these values to align with expected behaviors. Model calibration enables the practical application of ODE models in scenarios where not all parameters can be experimentally determined. To enhance the predictive accuracy of these models, there is an emphasis ...
MDS was a practical assessment instrument used to assess the status of magnesium deficiency. Multivariable logistic and restricted cubic spline models were used to assess the associations between MDS and depression. Results Among the 18247 adults, 1753 participants were diagnosed with depression. ...
and we demonstrated its utility on four single-cell RNA-seq datasets. We found that interpretable deep learning with KPNNs was practical even for large single-cell atlases, achieved high prediction accuracies for distinguishing between cell states, and uncovered characteristic regulatory differences for...
MC development occurs from a hematopoietic stem cell (HSC) in bone marrow that proceeds along the myeloid lineage through the common myeloid progenitor (CMP) and granulocyte/macrophage progenitor (GMP). Mast cell progenitors (MCP) develop either from GMP or directly from HSC, circulate in the ...