Devices were cut from the molds and holes for tubing connection (0.75 mm) and electrode insertion (1.5 mm) were created with biopsy punches, the devices were cleaned by application of Scotch tape and sonication in isopropanol (5 min). After oven drying, devices were bonded to glass ...
Sex has also been shown to influence the complexity and diversity of gut microbiome populations [30,31,32,33]. Using a panel of over 100 diverse inbred strains of mice, Org et al. identified distinct gut microbial communities in male vs. female mice whose composition was influenced by the p...
RNA sequencing (RNA-seq) has become a central method in biology and many technological variants exist that are adapted to different biological questions [1]. Its most frequent application is the quantification of gene expression levels to identify differentially expressed genes, infer regulatory networks...
Machine learning methods hold the promise to reduce the costs and the failure rates of conventional drug discovery pipelines. This issue is especially pressing for neurodegenerative diseases, where the development of disease-modifying drugs has been particularly challenging. To address this problem, we d...
Research participants provided autopsy consent limited to removal of the brain. All data were analyzed anonymously. Skin punches were performed following written informed consent from the donor. The informed consent was approved by the Washington University School of Medicine Institutional Review Board and...
(365 nm, 10 mW/cm2) for 30 s. A striped photomask was placed on top and gel was exposed to the second round of UV light for one minute. Biopsy punches were used to cut hydrogel in circle-shape, then the hydrogels were placed into well plates for cell seeding. SD2 control ...
Total RNA was extracted from tissue punches using the Allprep® DNA/RNA Micro Kit (Qiagen, Valencia, CA, USA) and assessed by chromatography (Agilent Bioanalyzer, Santa Clara, CA, USA). RIN was 8.35 ± 0.04 (mean ± standard error of the mean (SEM)), indicating excel-lent ...
The tissue taken for analysis was enriched for tumor cells after the evaluation of hematoxylin and eosin (H&E)-stained slides by a pathologist and another gynecologic pathologist to confirm histological diagnosis. Based on this evaluation, 2.0- or 2.5-mm tissue punches were taken from the ...
The Power of Soft Skills Transforming lab culture through effective communication and leadership Sponsored Our Water, Our Priority Overcoming Operational Bottlenecks in High-Throughput Clinical Labs Rolling with the Punches in the Cannabis Industry Weighing the Challenges and Breakthroughs in Quantitative MS ...
47 million punches to my body ( the description Darrel chooses to use to describe Vibration Training ) I have no current injuries at 45, weight 70 kg. which is a far cry from my sore knees, back spasms and lovely grinding noise my shoulder used to make at 35 ( 85kg ). I can now...